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https://eyecro.com

  • AboutUs_Normal-24 The EyeCRO Approach
    • About Us
    • Careers
    • Location
    • Partners
  • MiDrops MiDROPS™
  • InVivo Models
    • Allergic Conjunctivitis
    • Corneal Sensitivity
    • Corneal Wound Healing
    • Diabetic Keratopathy
    • DL-AAA Retinal Leakage
    • Dry Eye Disease
    • Endotoxin induced Uveitis
    • Experimental Autoimmune Uveitis
    • Geographic Atrophy
    • Inherited Retinal Degenerations
    • Ischemia Reperfusion Injury
    • Laser-induced Choroidal Neovascularization
    • Light Damaged
    • Mitochondrial Neuropathy
    • Optic Nerve Crush
    • Oxygen Induced Retinopathy
    • Retinal Detachment
    • Retinal Vein Occlusion
    • STZ-induced Diabetic Retinopathy
    • VEGF-induced permeability
  • InVitro Capabilities
    • A2E Quantification
    • Bioanalytical Detection
    • Biochemistry
    • Histology
    • Ophthalmic Imaging and Physiology
  • News News
  • ContactUs Contact Us

Preclinical Ophthalmic Contract Research

Laser-induced Choroidal Neovascularization

The Laser CNV model has been a reliable animal surrogate in the development of therapeutics to treat retinal diseases, such as Wet-AMD.  The pathogenesis of lesion development and regression appears to be conserved amongst lower mammals, as rodents have been largely predicative of efficacy observed in larger non-human primates, and subsequently in human clinical trials.  We sought to develop more robust and reproducible methods for Laser CNV using a Micron III small animal funduscope (Phoenix Research Inc.) which was attached to a thermal laser.  This system allows for the fine manipulation of laser focusing and allows the performance of true non-invasive fluorescein angiography without having to sacrifice the animals.  The same eyes can be used for lesion quantification at multiple timepoints and the eyes/tissue can be harvested at study termination for a secondary endpoint, such as ELISA, qRT-PCR, immunoblot, LC-MS/MS quantification of drug, or immunohistochemistry.  After examining numerous parameters, including rat strain, age, laser settings, and location of lesions, we developed optimal parameters to ensure successful laser application and penetration of the Bruch’s membrane to produce consistent lesions.  We have also identified and validated the use of a rat anti-VEGF antibody for use as a positive control which causes a significant reduction of lesions size and grade of leakage.

laser CNV baseline data

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