Corneal Neovascularization and Wound Healing

Corneal Neovascularization

Corneal neovascularization (NV) is characterized by the growth of new blood vessels into the normally avascular cornea. New blood vessels lead to lipid exudation, persistent inflammation, and scarring. This disrupts corneal transparency and visual acuity. Advanced cornea NV can become permanently vision-threatening and often contributes rejection corneal grafts after keratoplasty. In order to test this potential effect of therapeutic agents, mouse corneas are subjected to alkali burns with 1M NaOH. Clinical scoring is performed based on the opacity of the cornea, length of neovessels, and size of neovessels. Quantification of CV can be assess by immunostaining for neovessels with anti-CD31 or lymphogiogenesis with LYVE-1in whole cornea flat mounts.

Corneal Wound Healing

Corneal injuries are common in both men and women across all age groups and are among the most common eye conditions seen in emergency departments.(1) Corneal abrasions comprise eight percent of all eye presentations in primary.(2) in vivo corneal wound healing models are used. The mechanical injury model allows for the testing agents that may improve re-epithelialization. For this model, an Algerbrush II with a 0.5mm Burr or a dulled blade is used to remove the corneal epithelial layer. The speed of wound closure is determined by Fluorescein staining and the area of the wound is calculated by imaging software.  We also offer the alkali cornea burn model. To accomplish this, a 2mm filter paper circle is soaked in 0.1 to 1M NaOH for 10 to 30s. We also offer an in vitro corneal wound healing model that utilizes primary murine or human corneal epithelial cells. These models can be used for studying the effect of potential therapeutics on cell migration, cell proliferation, cell differentiation, barrier function, scarring, reinnervation, angiogenesis/ lymphangiogenesis

Primary Human Corneal Endothelial Cells

 

 

Mechanical Wound

 

Alkali Burn

 

1. Shields T, Sloane PD. A comparison of eye problems in primary care and ophthalmology practices.Fam Med.         1991;23(7):544–546.

2. Wong TY, Lincoln A, Tielsch JM, Baker SP. The epidemiology of ocular injury in a major US automobile corporation.Eye         (Lond). 1998;12(pt 5):870–874.

Preclinical Ophthalmic Contract Research