EyeCRO has established in vitro models of the nuclear translocation of hypoxia induced factor-1alpha (HIF-1α) and nuclear factor kappa B (NFқB) to explore whether new drugs affect the activation of key proteins in the pathogenesis of ocular diseases and cancer in ocular cells or cancer cells. The nuclear translocation of these proteins is detected by Immunocytochemistry method and Western blotting. In the chart 4 you can see that the compound effectively inhibits the protein HIF-1α. The immunflourescence makes the protein much easier to see.

Immunocytochemistry - figure 1Immunocytochemistry - figure 2

Preclinical Ophthalmic Contract Research