Flow Cytometry Analysis of Cell Cycle

Cell cycle and apoptosis

EyeCRO provides the analysis of cell cycle and apoptotic cells to determine whether the effect of new drugs is caused by slowing cell growth or inducing apoptosis. Endothelial and cancer cells were treated with new drugs for 24 h. After treatment, cells were fixed and stained with propidium iodide (PI). DNA content of cells was analyzed by flow cytometry. The percent of cells in each phase and apoptosis was analyzed by ModFit LT (Verity Software). The chart below shows the different stages of cellular growth. Chart A shows a flow cytometric analysis of untreated BRCEC cells. Chart B shows a flow cytometric analysis of BRCECs treated with 4 µM of a compound. Chart C data represents the percentage of cell distribution.

Preclinical Ophthalmic Contract Research