Cytokine Production

ICAM-1 & VEGF

EyeCro provides cell-based assays to determine the effect of new drugs on the expression of angiogenic stimulators and inflammatory cytokines, e.g. vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1). VEGF expression in human retinal capillary endothelial cells (HRCECs) and human retinal pigment epithelial cells (ARPE-19) is elevated by cobalt chloride (CoCl2). ICAM-1expression in ARPE-19 is stimulated by tumor necrosis factor-alpha (TNF-α). The VEGF and soluble ICAM-1 levels in cell-free conditioned medium of each treatment were measured by Enzyme-linked immunosorbent assay (ELISA). In chart 1 below the red bar represents a normal cell, and the blue bar represents a cell that is induced with angiogenic(left) and inflammatory(right) cytokines. By administering a compound that inhibits these cytokines you can see the results.

Preclinical Ophthalmic Contract Research